Project

Part:BBa_K2865003

Designed by: Chuqi Wang   Group: iGEM18_SMMU-China   (2018-09-30)


[AAV9]-Right-ITR

The Inverted Terminal Repeats (ITRs) are two 145-base sequences that locate on both sides of recombinant AAV (rAAV) shuttle vectors. They are named so because of their symmetry, which has been shown to be required for efficient multiplication of the AAV. We extracted both left and right ITRs through PCR and ligated them to each side of AAV-9 shuttle vectors respectively. AAV-9 virus particles were then packaged and applied in therapeutic use. This part (BBa_K2865002) encodes for the right ITR of AAV-9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 34
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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